Recombinant human SHP-1 protein

Recombinant Protein

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541) was cloned into an E. coli expression vector. The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). The recombinant SHP-1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid (Met) is attached at N-terminus.

Liquid in. 25mM Tris-HCl buffer (pH 7.5) containing 2mM beta-mercaptoethanol, 1mM EDTA 1mM DTT, 20%glycerol

Signal Transduction

< 1 EU per 1ug of protein (determined by LAL method)

Human

Protein tyrosine phosphatase non-receptor type 6 isoform 1, PTPN6, HCP, PTP1C, Tyrosine-protein phosphatase non-receptor type 6, EC 3.1.3.48, Protein-tyrosine phosphatase 1C, PTP-1C, Hematopoietic cell protein-tyrosine phosphatase, SH-PTP1, Protein-tyrosine phosphatase SHP-1, PTPN6, HCP, HCPH, SHP1, HPTP1C, SHP-1L EC 3.1.3.48, Protein tyrosine phosphatase non-receptor type 6 isoform 1, 70 kda SHP1L protein, Hematopoietic cell phosphatase, Hematopoietic cell protein tyrosine phosphatase, HPTP 1C, Protein tyrosine phosphatase 1C, Protein tyrosine phosphatase SHP 1, Protein tyrosine phosphatase SHP1, PTP 1C, PTPN 6, SH PTP 1, SH PTP1, SHP 1, SHP 1L, SHP1L, SHPTP 1, SHPTP1, Tyrosine protein phosphatase non receptor type 6