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HCoV-NL63 Nucleocapsid protein, His Tag European Partner

Item no. NUN-V5146-100ug
Manufacturer ACROBiosystems
Amount 100 ug
Quantity options 100 ug 500 ug x 2
Category
Type Proteins Recombinant
Format Solid
Specific against Coronavirus
Host E.coli
Conjugate/Tag Unconjugated, HIS
Purity 95%
Citations (1) Reuter M, et al. Virus Genes. 2018. 54(1):5-16.
(2) Grunewald ME, et al. Virology. 2018. 517:62-68.
(3) Jeeva S, et al. PLoS One. 2017. 12(9):e0184935.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Nucleocapsid protein,NP,Protein N,COVID-19
Shipping Condition Room temperature
Available
Specificity HCoV-NL63
Manufacturer - Category
Protein / Viral Proteins
Manufacturer - Conjugate / Tag
N-6xHis, Unconjugated
Shipping Temperature
RT
Storage Conditions
-20°C
Molecular Weight
43.2 kDa
Manufacturer - Format
Powder
Description
HCoV-NL63 Nucleocapsid, His Tag (NUN-V5146) is expressed from E. coli cells. It contains AA Met 1 - His 377 (Accession # Q6Q1R8-1).
Background
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
Molecule
Nucleocapsid protein
Exp Region
Met 1 - His 377
Characteristics
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 43.2 kDa. The protein migrates as 50 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin
1.0 EU per μg
Buffer
40 mM PB, 500 mM NaCl, pH 7.4
Stability
● -20°C to -70°C for 12 months in lyophilized state;
● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant
trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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