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Recombinant Human Wnt3a/Wnt-3a

Recombinant Human Wnt3a/Wnt-3a

Item no.	PKSH033972-10
Manufacturer	Elabscience
Amount	10 ug
Quantity options	10 ug 50 ug
Category	Neuroscience Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Liquid
Applications	Cell Culture
Specific against	Human (Homo sapiens)
Host	HEK293 cells
Conjugate/Tag	Untagged
Purity	> 95 % as determined by reducing SDS-PAGE.
Sequence	Ser19-Lys352
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	MGC119418, MGC119419, MGC119420, Wnt-3a, Wnt3a, member 3A, protein Wnt-3a, wingless-type MMTV integration site family
Shipping Condition	Cool pack
Available
Manufacturer - Category	Recombinant Proteins / Cytokines
Manufacturer - Conjugate / Tag	None
Shipping Temperature	This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions	Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight	58.5 kDa
Observed Molecular Weight	66-73 kDa
Background	Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes.Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).
Abbreviation	Wnt3a; Wnt-3a
Endotoxin	< 0.01 EU per μg of the protein as determined by the LAL method.
Reconstitution	Please refer to the printed manual for detailed information.
Formulation	Lyophilized from a 0.2 um filtered solution of 10Mm PB .5% Sucrose, 0.01% Tween80 pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Activity	Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The ED50 for this effect is 71.45 ng/ml.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

PKSH033972-10-datasheet.pdf

PKSH033972-10-safety-datasheet.pdf