Comparison

Recombinant SARS-CoV-2 (SARS-CoV-2) S protein RBD (C-mFc-6His tag)

Item no. PKSV030276-100
Manufacturer Elabscience
Amount 100 ug
Quantity options 100 ug 50 ug
Category
Type Proteins Recombinant
Format Lyophilized Powder
Applications ELISA
Specific against SARS-CoV-2
Host HEK293 cells
Conjugate/Tag C-mFc, HIS
Purity > 95 % as determined by reducing SDS-PAGE.
Sequence Arg319-Phe541
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias COVID-19, S glycoprotein RBD, S protein RBD, Spike glycoprotein Receptor-binding domain, Spike protein RBD
Shipping Condition Cool pack
Available
Manufacturer - Category
Recombinant Proteins / Viral Proteins
Shipping Temperature
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
52.2 kDa
Background
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.
Abbreviation
Spike
Endotoxin
Please contact us for more information.
Reconstitution
Please refer to the printed manual for detailed information.
Formulation
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Activity
1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human S-RBD, mFc-His tagged protein (PKSV030276) can bind Anti-SARS-CoV-2 Neutralizing antibody(E-AB-V1028) in a linear range of 0.24-15.62 ng/ml.
2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) S-RBD, mFc-His tagged protein (PKSV030276) can bind Human ACE2, hFc Tagged protein(PKSR030492) in a linear range of 0.488-49.83 ng/ml.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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