« Back
Home /
Mouse SEMA3A/Semaphorin-3A Protein (SEM-MM13A)

Mouse SEMA3A/Semaphorin-3A Protein (SEM-MM13A)

Item no.	SEM-MM13A-100UG
Manufacturer	KactusBio
Amount	100 ug
Quantity options	100 ug 500 ug
Category	Neuroscience Molecular Targets for Neuroscience Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Liquid
Specific against	Mouse (Murine, Mus musculus)
Host	HEK293
Conjugate/Tag	HIS
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Formula	Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 100mM L-arginine, 100mM glutamic acid (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization.
Dry ice	Yes
Sequence	Tyr21-Val772
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Sema III,Semaphorin,Sema D,coll-1,collapsin 1,Hsema-I,Hsema-III, SEMA1,Sema3A,SEMAD,SEMAIII,SEMAL,Semaphorin 3A
Shipping Condition	Dry ice
Available
Manufacturer - Type	Recombinant Protein
Manufacturer - Targets	SEMA3A/Semaphorin-3A
Manufacturer - Conjugate / Tag	N-His
Shipping Temperature	Shipped with dry ice.
Storage Conditions	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Molecular Weight	The protein has a predicted MW of 86.6 kDa. Due to furin cleavage site, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result.
Description	Recombinant Mouse SEMA3A/Semaphorin-3A Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Tyr21-Val772.
Background	The CRMP proteins were originally identified as mediators of Sema3A signaling and neuronal differentiation. Much has been learned about the mechanism by which CRMPs regulate cellular responses to Sema3A.The secreted protein Sema3A (collapsin-1) was the first identified vertebrate semaphorin. Sema3A acts primarily as a repulsive axon guidance cue, and can cause a dramatic collapse of the growth cone lamellipodium.Neuropilin-1 (NP1) and members of the class A plexins (PlexA) form a Sema3A receptor complex, with NP1 serving as a high-affinity ligand binding partner, and PlexA transducing the signal into the cell via its large intracellular domain.
Endotoxin	Less than 1 EU per μg by the LAL method.
Formulation	Supplied as 0.22 μm filtered solution in PBS, 200 mM L-Argine (pH 7.4).
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Biotinylated	no

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

SEM-MM13A-100UG-datasheet.pdf

SEM-MM13A-100UG-safety-datasheet.pdf