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Human Siglec-7 / CD328 Protein, Fc (L117A, L118A) Tag, low Endotoxin (MALS verified)

Human Siglec-7 / CD328 Protein, Fc (L117A, L118A) Tag, low Endotoxin (MALS verified)

Item no.	SI7-H5258-50ug
Manufacturer	ACROBiosystems
Amount	50 ug
Quantity options	500 ug 50 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Solid
Specific against	Human (Homo sapiens)
Host	HEK293
Conjugate/Tag	Unconjugated, Fc
Purity	95%
NCBI	NP_055200.1
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CDw328,D-siglec,A79 membrane protein,p75,Adhesion inhibitory receptor molecule 1,AIRM-1
Shipping Condition	Room temperature
Available
Manufacturer - Category	Protein / Bio-Markers & CD Antigens
Manufacturer - Conjugate / Tag	C-Fc(A2), Unconjugated
Shipping Temperature	RT
Storage Conditions	-20°C
Molecular Weight	63.3 kDa
Manufacturer - Format	Powder
Description	Human Siglec-7, Fc (L117A, L118A) Tag (SI7-H5258) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Leu 353 (Accession # Q9Y286-1).
Background	Siglec-7 is a member of the human CD33‐related Siglec receptor. The extracellular region of Siglec‐7 is characterized by an N‐terminal V‐set Ig domain that can bind sialic acid and two C2‐set Ig domains. The cytoplasmic tail of Siglec‐7 has one immune‐receptor tyrosine‐based inhibitory motif (ITIM) and one ITIM‐like motif. Siglec-7 is considered as a sialic acid-dependent immunoreceptor with inhibitory potential and expressed predominantly on human NK cells, monocytes and a small subset of CD8+ T cells.
Molecule	Siglec-7
Exp Region	Gln 19 - Leu 353
Characteristics	This protein carries a Fc (L117A, L118A) tag at the C-terminus. The protein has a calculated MW of 63.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Mutations (L117A, L118A/EU number: L234A/L235A) in human immunoglobulin G1 (hIgG1) Fc diminish binding to FcγRI leading to a significant reduction in ADCC and CDC in comparison to wild type human IgG1.
Endotoxin	0.01 EU per μg
Buffer	25 mM MES, 150 mM NaCl, pH 5.5
Stability	● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant	trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

SI7-H5258-50ug-datasheet.pdf

SI7-H5258-50ug-safety-datasheet.pdf