Comparison

HCoV-NL63 S protein, His Tag European Partner

Item no. SPN-H52H4-50ug
Manufacturer ACROBiosystems
Amount 50 ug
Quantity options 500 ug 50 ug
Category
Type Proteins Recombinant
Format Solid
Specific against Coronavirus
Host HEK293
Conjugate/Tag Unconjugated, HIS
Purity 90%
NCBI YP_003767.1
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Spike,S protein,Spike glycoprotein,S glycoprotein
Shipping Condition Room temperature
Available
Specificity HCoV-NL63
Manufacturer - Category
Protein / Viral Proteins
Manufacturer - Conjugate / Tag
C-10xHis, Unconjugated
Shipping Temperature
RT
Storage Conditions
-20°C
Molecular Weight
146.9 kDa
Manufacturer - Format
Powder
Description
HCoV-NL63 Spike Trimer, His Tag (SPN-H52H4) is expressed from human 293 cells (HEK293). It contains Phe 16 - Asn 1291 (Accession # Q6Q1S2-1).The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the N-terminus.
Background
Human coronavirus NL63 (HCoV-NL63) is one of seven known coronaviruses to infect humans. The virus is an enveloped, positive-sense, single-stranded RNA virus which enters its host cell by binding to ACE2. Infection with the virus has been confirmed worldwide, and has an association with many common symptoms and diseases. The spike protein of HCoV-NL63 is a trimer protruding from the viral membrane to engage cellular receptors and mediate viral fusion with host membranes. Each spike trimer contains two large regions: N-terminal S1 responsible for receptor binding and C-terminal S2 responsible for fusion.
Molecule
Spike protein
Exp Region
Phe 16 - Asn 1291
Characteristics
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 146.9 kDa. The protein migrates as 200-240 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
1.0 EU per μg
Buffer
0.1 M Sodium citrate, pH 5.5
Stability
● -20°C to -70°C for 12 months in lyophilized state;
● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant
trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 ug
Available: In stock
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