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HCoV-HKU1 (isolate N5) Spike Trimer Protein, His Tag (MALS verified)

HCoV-HKU1 (isolate N5) Spike Trimer Protein, His Tag (MALS verified)

Item no.	SPN-H52H5-500ug
Manufacturer	ACROBiosystems
Amount	500 ug
Quantity options	500 ug 50 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Solid
Specific against	Coronavirus
Host	HEK293
Conjugate/Tag	Unconjugated, HIS
Purity	95%
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Spike,S protein,Spike glycoprotein,S glycoprotein
Shipping Condition	Room temperature
Available
Specificity	HCoV-HKU1
Manufacturer - Category	Protein / Viral Proteins
Manufacturer - Conjugate / Tag	C-10xHis, Unconjugated
Shipping Temperature	RT
Storage Conditions	-20°C
Molecular Weight	145.5 kDa
Manufacturer - Format	Powder
Description	HCoV-HKU1 (isolate N5) Spike Trimer, His Tag (SPN-H52H5) is the ectodomain of HCoV-HKU1 (isolate N5) spike protein which contains AA Ala 13 - Asn 1276 (Accession # Q0ZME7-1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. The S1/S2 furin-recognition site 752-RRKRR-756 is mutated to GGSGS to generate the uncleaved protein. Proline substitutions (N1067P, L1068P) are introduced to stabilize the trimeric prefusion state of the spike protein.
Background	It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Molecule	Spike protein
Exp Region	Ala 13 - Asn 1276
Characteristics	This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 145.5 kDa. The protein migrates as 180-210 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin	1.0 EU per μg
Buffer	0.1 M Sodium citrate, pH 5.5
Stability	● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant	trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

SPN-H52H5-500ug-datasheet.pdf

SPN-H52H5-500ug-safety-datasheet.pdf