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Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer Protein, His,Avitag™ (MALS verified) European Partner

Item no. SPN-H82E3-200ug
Manufacturer ACROBiosystems
Amount 200 ug
Quantity options 200 ug 25 ug
Category
Type Proteins Recombinant
Format Solid
Specific against Coronavirus
Host HEK293
Conjugate/Tag Biotin, HIS, Avi
Purity 95%
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Spike,S protein,Spike glycoprotein,S glycoprotein
Shipping Condition Room temperature
Available
Specificity HCoV-HKU1
Manufacturer - Category
Protein / MABSol® Biotin Labeled Proteins
Manufacturer - Conjugate / Tag
C-10xHis & C-Avi, Biotin-labeled
Shipping Temperature
RT
Storage Conditions
-20°C
Molecular Weight
147.2 kDa
Manufacturer - Format
Powder
Description
Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer, His, Avitag (SPN-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 13 - Asn 1276 (Accession # Q0ZME7-1). Amino acid substitutions RRKRR 752-756 GGSGS and NL 1067-1068 PP are introduced to abolish the furin cleavage site and stabilize the trimeric prefusion state of the spike protein, respectively.
Background
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Molecule
Spike protein
Exp Region
Ala 13 - Asn 1276
Characteristics
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 147.2 kDa. The protein migrates as 200-220 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
1.0 EU per μg
Buffer
0.1 M Sodium citrate, pH 5.5
Stability
● -20°C to -70°C for 12 months in lyophilized state;
● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant
trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 200 ug
Available: In stock
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