SN50, NF-kB Cell-Permeable Inhibitor Peptide (AAVALLPAVLLALLAPVQRKRQKLMP)

Blue Ice

2781, 5

Supplied as a lyophilized powder in trifluoroacetate salt.

Contains the nuclear localization sequence (NLS) of the transcription factor NF-kB p50 linked to the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF). The N-terminal K-FGF h-region confers cell-permeability, while the NLS (360-369) inhibits translocation of the NF-kB active complex into the nucleus. In murine endothelial LE-II cells induced by LPS, NF-kB nuclear translocation is maximally inhibited at 18uM.

Formula:
C₁₂₉H₂₃₀N₃₆O₂₉S

Sequence:
H-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Val-Gln-Arg-Lys-Arg-Gln-Lys-Leu-Met-Pro-OH

Solubility:
H2O (5mg/ml)

Molecular Weight:
2781.5

Purity:
≥97% (HPLC)

Form:
Supplied as a lyophilized powder in trifluoroacetate salt.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.