Anti-IL-8 / CXCL8 Antibody, Biotinylated

ELISA (dilution: 0.1, 0.2ug/ml), Western Blotting

Ambient temperature

Rabbit Anti-Human IL-8 / CXCL8 [+Biotin]

Phosphate buffered saline

Purified IgG conjugated to Biotin - lyophilized

Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution.. For long term storage the addition of 0.09% sodium azide is recommended.

This product is specific for human IL-8 (Interleukin-8), a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1(NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.

Antisera to human IL-8 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.