Nuclear Extraction Kit

Molecular Biology|Nucleic Acid Assays|Extraction & Purification

-20°C

N
E Reagents-I, -I
I, and -III
Protease Inhibitor Cocktail
Phosphatase Inhibitor Cocktail I
Phosphatase Inhibitor Cocktail I
I

Prepare all reagents and samples as instructed in the manual.
Add appropriate amount of 1
X N
E Reagent-I for sample amount
Culture Media: Disperse Cells
Tissue Samples: Homogeize using dounce homogenizer or tissue grinder
Incubate 15 minutes on ice
Add appropriate amount of 1
X N
E Reagent-I
I for sample amount
Incubate 2 minutes on ice
Centrifuge tubes at 4°C, 14000 x g for 30 seconds in microcentrifuge.
Transfer supernatant (cytoplasm fraction) into new tube. Store at -80°C
Add appropriate amount of 1
X N
E Reagent-III for sample amount
Vortex on highest setting for 10 seconds to suspend pelletes completly
Incubate for 40 minutes on ice, vortexing for 10 seconds every ten minutes
Centrifuge tubes at 4°C, 14000 x g for 10 minutes in microcentrifuge. Transfer supernatant (nuclear fraction) to a new tube and store at 0-80°

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