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Human/Mouse/Rat Phospho-ERK1/2, JNK, p38 MAPK Cell-Based ELISA Kit

Human/Mouse/Rat Phospho-ERK1/2, JNK, p38 MAPK Cell-Based ELISA Kit

Item no.	CBEL-ERK-SK
Manufacturer	Raybiotech
Amount	2 Plate Kit
Category	Oncology Neuroscience Molecular Targets for Neuroscience Neural Signaling By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Colorectal Cancer Breast Cancer
Type	Elisa-Kit
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Hamster - Armenian
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	JNK, Erk2, p38, Erk1
Shipping Condition	Cool pack
Available
Manufacturer - Category	Assay Kits\|Phosphorylation Assays\|ELISA Kits\|Cell-Based ELISA
Manufacturer - Targets	ERK1/2, JNK, p38
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Description	RayBio® Cell-Based Human/Mouse/Rat ERK1/2 (Thr202/Tyr204), JNK (Thr183/Tyr185), p38 MAPK (Thr180/Tyr182) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.
Compatible Sample Types	Adherent Cell Culture
Design Principle	Cell-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Gene Symbols	MAPK3, ERK1, ERK2, PRKM1, PRKM3/MAPK1, PRKM2/MAPK8, JNK1, PRKM8, SAPK1, SAPK1C/MAPK14, CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A
Kit Components	Cell Culture Microplate Wash Buffer A Wash Buffer B Fixing Solution HRP-Conjugated Secondary Antibody Blocking Buffer TMB One-Step Substrate Stop Solution Quenching Buffer
Other Materials Required	Distilled or deionized water 100 ml and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors Precision pipettes to deliver 2 µl to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation Benchtop rocker or shaker Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline	Seed 10, 000-30, 000 cells into each well and incubate overnight. Apply various treatment, inhibitors or activators according to manufacture's instructions. Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking. Add 200 µl of prepared 1 X Quenching Buffer and incubate 20 min at RT. Add 200 µl of Blocking Solution and incubate for 1 h at 37 °C. Add 50 µl of 1 X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT. Add 50 µl of prepared 1 X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Site and signal pathway-specific In vitro detection of adherent cell culture No sample lysis needed Compatible with a standard ELISA plate reader Faster results than with ELISA Adaptable for high-throughput screening and drug discovery
UNSPSC Code	41116158
Protein Name & Synonyms	Mitogen-activated protein kinase 3 (MAP kinase 3) (MAPK 3) (EC 2.7.11.24) (ERT2) (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin-stimulated MAP2 kinase) (MAP kinase isoform p44) (p44-MAPK) (Microtubule-associated protein 2 kinase) (p44-ERK1)/Mitogen-activated protein kinase 1 (MAP kinase 1) (MAPK 1) (EC 2.7.11.24) (ERT1) (Extracellular signal-regulated kinase 2) (ERK-2) (MAP kinase isoform p42) (p42-MAPK) (Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2)/Mitogen-activated protein kinase 8 (MAP kinase 8) (MAPK 8) (EC 2.7.11.24) (JNK-46) (Stress-activated protein kinase 1c) (SAPK1c) (Stress-activated protein kinase JNK1) (c-Jun N-terminal kinase 1/Mitogen-activated protein kinase 14 (MAP kinase 14) (MAPK 14) (EC 2.7.11.24) (Cytokine suppressive anti-inflammatory drug-binding protein) (CSAID-binding protein) (CSBP) (MAP kinase MXI2) (MAX-interacting protein 2) (Mitogen-activated protein kinase p38 alpha) (MAP kinase p38 alpha) (Stress-activated protein kinase 2a) (SAPK2a)
Manufacturer - Specificity	The antibodies provided in this kit recognizes human, mouse and rat Erk1 phosphorylated at sites Thr202/Tyr204, Erk2 phosphorylated at sites Thr185/Tyr187, JNK phosphorylated at sites Thr183/Tyr185 and p38 phosphorylated at sites Thr180/Tyr182. This kit also recognizes total Erk1/2, total JNK and total p38 for comparison.
Pathway	MAPK Signaling

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

CBEL-ERK-SK.pdf

CBEL-ERK-SK-datasheet.pdf

CBEL-ERK-SK-safety-datasheet.pdf