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Recombinant Human INTERLEUKIN-1RA

Recombinant Human INTERLEUKIN-1RA

Manufacturer	Raybiotech
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human
Amount	20 ug
Item no.	229-20161
eClass 6.1	34160400
eClass 9.0	42020190
Available
Introduction	Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring protein which inhibits the activity of IL-1 alpha and IL-1 beta by competitively blocking binding to cell surface receptors for IL-1. In an assay of dose dependent inhibition of IL-1 stimulation of D10S cells, the ED50 was expected to be 20-40ng/ml.
Protein Name	IL-1RA
Molecular Weight (kDa)	17kD/153 amino acids
Purity	>98% by SDS PAGE
Concentration	Total protein concentration ca.0.1mg/ml after reconstitution.
Preservative	None present
Endotoxin Level	<0.1ng/ug
Buffers	TRIS buffered saline
Protein Type	Recombinant Protein
Recommended Applications	ELISA (0.2-0.4ng/well), Functional Assays (20-40ng/ml), Western Blotting (1.5-3.0ng/lane)
Reconstitution	Reconstitute with 0.2ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommends that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum albumin (BSA) is recommended.
Storage/stability	Prior to reconstitution store at +4° C. Following reconstitution store at -20° C. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.