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Recombinant Human Wilms Tumor 1 Protein, HEK293 Lysate

Recombinant Human Wilms Tumor 1 Protein, HEK293 Lysate

Item no.	230-10035-100
Manufacturer	Raybiotech
Amount	100 ug
Category	Oncology By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Myeloma Sarcoma Peptides & Proteins Recombinant Proteins Mesothelioma Immunoprecipitation Western Blot ELISA By Organ Testicular & Prostate Cancer Renal Cancer Immunostaining Dot Blot
Type	Proteins Recombinant
Format	Lyophilized
Applications	WB, IP, ELISA, Dot
Specific against	Human (Homo sapiens)
Host	HEK293 cells
Conjugate/Tag	HIS
Purity	Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Western blotting control, antibody validation (i.e., hybridoma screening, antibody pair screening), ELISA, EIA, dot blot, immunoprecipitation (IP), protein array, protein-protein interaction studies, post-translational modifications
Manufacturer - Category	Proteins\|Recombinant Proteins\|Human Proteins
Manufacturer - Conjugate / Tag	N-terminal 6x histidine tag
Shipping Temperature	Ambient temperature
Storage Conditions	-20°C
Molecular Weight	36.8 kDa
Description	Recombinant human Wilms Tumor 1 overexpressed in cell lysate, derived from transfected HEK293 cells (100 µg). Purchase will also include one vial of normal control HEK293 cell lysate (Catalog # 230-10006) transfected with empty expression vector.
Gene Symbols	WT1
UNSPSC Code	12352202
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS containing mammalian cell protease inhibitor cocktail.
Expressed Region	Glu2-Leu302
Protein Name & Synonyms	Wilms tumor 1, WT1
Format	Lyophilized powder
Purification	Unpurified overexpression cell lysate
Concentration	Determined by BCA protein assay kit (Thermo Scientific)
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level	Not determined
Preservative	None

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

230-10035-100.pdf

230-10035-100-datasheet.pdf

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Amount: 100 ug

available

Delivery expected until 10/9/2025

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