Recombinant Human Matrix Metalloproteinase-9 (MMP-9), transfected HEK293 cell culture supernatant

Western blotting control, antibody validation (i.e., hybridoma screening, antibody pair screening), ELISA, EIA, dot blotting, immunoprecipitation (IP), protein array, protein functional assay, protein-protein interaction, post-translational modifications, etc.

N-terminal histidine tag

-20°C

MMP9

Culture supernatant of transfected HEK293 cells

Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, Matrix Metallopeptidase 9, CLG4B, Matrix Metallopeptidase 9, Gelatinase B, Macrophage Gelatinase, Type V Collagenase, MandP2.

Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80 °, C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

Not determined