Recombinant Human CD318 (CDCP1) Protein

Proteins|Recombinant Proteins|Human Proteins

Blue ice

Recombinant Human CD318/CDCP1 has a calculated molecular mass of 72 kDa. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs).

CDCP1

Culture supernatant of transfected HEK293 cells

CD318, CUB Domain Containing Protein 1, Subtractive Immunization M Plus HEp3-Associated 135 KDa Protein, Transmembrane And Associated With Src Kinases, Membrane Glycoprotein Gp140, SIMA135, TRASK, CD318 Antigen, CD318

Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80 °C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.