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Recombinant Rat INTERLEUKIN-1 BETA

Recombinant Rat INTERLEUKIN-1 BETA

Manufacturer	Raybiotech
Category	Neuroscience Neuroinflammation Metabolism Cholesterol & Lipid Metabolism Peptides & Proteins Recombinant Proteins Cell Biology Cell Movement Cell Adhesion Cell Culture Cytokines
Type	Proteins
Specific against	Rat
Amount	10 ug
Item no.	229-20326
Targets	IL1B
eClass 6.1	34160400
eClass 9.0	42020190
Available
Description	229-20326 is an E. coli-derived recombinant protein corresponding to full-length mature rat interleukin 1 beta (IL-1b), an immunomodulary cytokine that mediates a wide range of immune responses.
Protein Name	IL-1 BETA
Molecular Weight (kDa)	17.3kD (153 Amino acid sequence)
Purity	>98% by SDS PAGE and HPLC analysis
Formulation	10mM Sodium Acetate pH6.5
Concentration	Total protein concentration ca.0.1 mg/ml after reconstitution
Preservative	None present
Endotoxin Level	<0.1 ng/ug
Protein Type	Recombinant Protein
Recommended Applications	ELISA (0.2-0.4ng/well), Functional Assays (0.1-10ng/ml), Western Blotting (1.5-3.0ng/lane)
Biological activity	129-20326 has a specific activity of > 1 x 10⁷ units/mg as determined by dose-dependent stimulation of proliferation of murine D10S cells.
Reconstitution	Reconstitute with 0.1 ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommends that the vial is gently mixed after reconstitution.
Storage/stability	Prior to reconstitution store at +4° C. Following reconstitution store at -20° C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.