5-Bromo-6-chloro-3-indolyl b-D-galactopyranoside (Magenta gal)

RT

408, 63

White to off-white powder

5-Bromo-6-chloro-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-D-galactosidase, yielding a magenta precipitate and contained in a chromogenic culture media. It is an indolyl carbohydrate that is the alpha-D-mannoside of indoxyl, in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. When Magenta-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Red-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

Synonyms:
Red b-D-gal; Magenta gal

CAS No:
93863-88-8

Molecular Formula:
C14H15BrClNO6

Molecular Weight:
408.63

Appearance:
White to off-white powder

Purity:
≥98% (HPLC)

FT-IR Spectrum:
Conforms to reference

Solubility:
Methanol

Storage and Stability:
May be stored at RT for short-term only. Long-term storage is recommended at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.