Fluorescein Diphosphate Tetraammonium Salt (FDP)

RT

560, 39

Yellow solid

Upon interaction with phosphatases the colorless and non-fluorescent FDP is hydrolyzed to highly fluorescent fluorescein, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments that are equipped with the Argon laser excitation. Alternatively, FDP can also be used to detect phosphatases in a chromogenic mode since the enzymatic product (fluorescein) exhibits a large extinction coefficient (close to 100, 000 cm-1mol-1). In some literature, FDP was considered to be one of the most sensitive fluorogenic phosphatase substrates. FDP has been widely used in various ELISA assays. Additionally it is also used to detect tyrosine phosphatases. FDP is thermally unstable, and special cautions need be excised for storing the solid sample and stock solutions.

Synonyms:
FDP

CAS Number:
217305-49-2

Molecular Formula:
C₂₀H₂₆N₄O₁₁P₂

Molecular Weight:
560.39

Appearance:
Yellow solid

Purity:
≥98%

Excitation:
498nm

Emission:
517nm

Absorbance:
487nm

Extinction Coefficient (cm-1 M-1):
80000

Quantum Yield:
0.7900

Correction Factor (260nm):
0.32

Correction Factor (280nm):
0.275

Solubility:
Water

Storage and Stability:
Store at -20°C under inert atmosphere. Light sensitive. For maximum recovery of product, centrifuge the original vial prior to removing the cap.