Thiazolyl Blue Tetrazolium Bromide (MTT)

RT

414, 32

Supplied as a pale yellow to yellow powder

Used as a membrane-permeable dye and is reduced by mitochondrial enzymes to measure cell proliferation.

Synonyms:
3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide
Methylthiazoletetrazolium; (4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyl-2H-tetrazolium bromide; MTT;
Methylthiazolyldiphenyl-tetrazolium bromide

CAS Number:
298-93-1

Molecular Weight:
414.32

Molecular Formula:
C₁₈H₁₆N₅SBr

Appearance:
Supplied as a pale yellow to yellow powder

Purity:
≥98%

Melting Point:
190-210°C

Water:
≤0.5%

Solubility:
DMSO (Slightly), Methanol (Slightly)

Storage and Stability:
Store at 4°C. Hygroscopic. Store under inert atmosphere. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Product is stable for 6 months from time of receipt.