Agarose High EEO

Molecular Biology / MB-BioSep Agarose

RT

White, fine, homogeneous, free flowing powder

High EEO Agarose is a general use agarose suitable for many nucleic acid and protein electrophoresis protocols.

Applications:
• Immunoelectrophoresis
• Serum protein electrophoresis
• Analytical DNA Gels ≥ 1000bp
• Blotting Assays (Southern, northern)
• For use with all buffer systems

EEO:
0.23-0.26

Loss on Drying:
≤7.0%

Melting Point (1.5%):
86.5-89.5°C

Gel Point (1.5%):
34.5-37.5°C

Gel Strength (1.5%):
≥1000g/cm2

Sulfate:
≤0.25%

Ash:
≤1.1%

Clarity (1.5%):
≤40 NTU

pH (1.5% gel):
As Reported

Colorimetry (1.5%):
As Reported

RNase:
None Detected

DNase:
None Detected

Quality Control:
Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.

Functional and Molecular Biology Testing: To Pass Test
• Comparative assay of different size DNA fragments
• Southern Blot (125-23, 000bp)
• Background Fluorescence (TAE/TE, EtBr):
• DNA Binding (TAE, EtBr)
• Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase)

Storage and Stability:
Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use.