2’-Deoxythymidine-5’-Triphosphate, Sodium Salt (dTTP) Solution, BioGenomics™

Biochemicals / Biochem-Nucleotides, Derivatives

-20°C

Molecular Biology Grade

PCR Grade nucleotides are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Thymidine 5′-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques.

dTTP, Molecular Biology Grade, is a 100mM clear, colorless solution of the sodium salt (pH 8.3).

Synonyms:
Thymidine 5'-(Tetrahydrogen Triphosphate) Sodium Salt; Thymidine-5’-Triphosphate, Sodium Salt; dTTP; 2'-Deoxythymidine 5'-triphosphate trisodium salt

Usage:
250ul at 100mM = 6, 250 standard reactions
1, 250ul at 100mM = 31, 250 standard reactions

CAS No:
18423-43-3
Free Acid: 365-08-2

Purity:
≥99% (HPLC)

Appearance:
Supplied as a liquid

dNDP:
≤0.9%

pH:
8.3

Quality Control:
Functionally tested with Sequenase, Taq DNA Polymerase, T7 DNA Polymerase and Pfu DNA Polymerase.

Concentration:
100mM

Lambda Max:
267±2nm

A250/A260:
0.65 ± 0.03

A280/A260:
0.73 ± 0.03

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

™The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffman-La Roche.