Fluorescein Maleimide

Molecular Biology / MB-Fluorescent Labels

-20°C

Highly Purified

Fluorescein-maleimide is a fluorescein dye linker containing a maleimide group. Maleimides can react with thiol-bearing compounds or biomolecule between pH 6.5 and 7.5 to produce thioester bonds. Fluorescein Maleimide is designed for fluoresceinating proteins, nucleic acids or other molecules containing one or more thiol groups. Molecules to be labeled with Fluorescein Maleimide require free thiol groups. In the case of proteins, Fluorescein Maleimide will react with exposed cysteine residues. Alternatively, free thiols may be generated by reducing disulfide bonds or by modifying other reactive groups such as primary amines with compounds like Traut's reagent (2-iminothiolane). For labeling nucleic acids, thiols can be introduced into DNA, RNA, or oligonucleotides using the FastTag, 5’ EndTag or 3’ EndTag nucleic acid labeling systems.
This fluorescent label can be directly detected or used as a hapten and detected with our high affinity antibody reagents.

Synonyms:
Fluorescent-Mal; 5-FITC-Maleimide; 5-FITC-Maleimide; Fluorescent-Mal

Molecular Weight:
529.52

Molecular Formula:
C27H19N3O7S

Purity:
≥95%

Appearance:
Orange yellow solid

Excitation:
494nm

Emission:
517nm

1H NMR:
Conforms to structure

Storage and Stability:
Lyophilized powder is stable for 12 months after receipt at -20°C. Light sensitive