Phosphatase, Alkaline, Calf Intestine, Solution (~3000u/mg)

Blue Ice

Molecular Biology Grade

Alkaline Phosphatase is used to dephosphorylate dsDNA and RNA in molecular cloning and DNA sequencing reactions.

Source:
Calf Intestine
Prepared from US origin starting material and collected in USDA approved facilities

Protein Concentration:
1mg/ml

Assay Method:
Based on the method of Garen and Levinthal whereby the reaction velocity is derived by measuring the absorbance increase at 410nm after hydrolysis of p-nitrophenyl phosphate.

Form:
Supplied as a liquid in deionized water.

Activity:
~3000 units/mg protein (glycine units). 1 glycine unit = 2-3 DEA units

Unit Definition:
One unit of enzyme hydrolyzes 1umole of p-nitrophenyl phosphate per minute at 37°C, pH 9.8 in the presence of Mg and Zn.

Quality Control:
SDS-PAGE
No contaminating RNase, DNase or Proteases detected

Storage and Stability:
Stable for 1 year at 2-8ºC