S1 Nuclease, A. orzyae, ss-DNA degradation, Solution

Blue Ice

34

Supplied as a liquid in 30mM sodium acetate, pH 4.6, 50mM sodium chloride, 1mM zinc chloride, 50% glycerol.

S1 Nuclease degrades single-stranded DNA and RNA yielding 5’-phosphoryl-terminated products.

Applications:
• Removal of protruding single-stranded termini in double-stranded DNA.
• Selective cleavage of single-stranded DNA
• Mapping RNA transcripts

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.