FCM Staining Buffer (BSA) (10X)

Assay Substrates and Reagents>Blocking Buffers|Cytometry Workflow Solutions

This FCM or BSA Stain Buffer can be used for the immunofluorescent staining of single-cell suspensions
prepared from either lymphoid tissues, bone marrow, peripheral blood, or cultured cells. This buffer is also
useful for applications using fluorescent reagents as well as for the suspension, washing, and storage of
cells destined for flow cytometric analysis (or fluorescence microscopy).
Application:
FCM Stain Buffer (BSA) is useful for staining cells with biotinylated antibodies and fluorochrome-
conjugated avidins because this staining medium contains no biotin. When present in staining media, free
biotin can interfere with the binding of fluorescent-avidins to biotinylated antibodies that have complexed
with their cognate, cell-associated antigens. Moreover, free biotin can bind to cells and contribute to an
increase in the non-specific, background staining of cells that are exposed to fluorochrome-conjugated
avidins. As such, FCM Stain Buffer (BSA) is designed to maintain cell viability and maximize fluorescence
signal intensities generated by pH-sensitive fluorochromes, such as FITC. The FCM Stain Buffer (BSA)
should be used at a 1X concentration.