TMB HRP Microwell Substrate Slow Kinetic One Component

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TMB HRPO Microwell Slow Kinetic Substrate (3, 3’, 5, 5’ tetramethylbenzidine) is a soluble substrate used with the
enzyme horseradish peroxidase (HRPO) designed for various qualitative and quantitative immunoassays but not
recommended for membrane or immunohistochemical applications where a precipitating reaction product is
required. Initially, the substrate should be colorless or slightly yellow in color and will be stored in a mildly acidic
buffer. TMB HRPO Microwell Slow Kinetic Substrate turns a deep blue color when reacted with horseradish
peroxidase labeled conjugates with absorbencies at 370 nm or in a range of 620 nm to 650 nm. The color is
changed to a bright yellow if an acidic stop reagent such as HCl or sulfuric acid is used. The absorbance should
be read at 450 nm if the reaction is stopped which increases the sensitivity 2-4 fold.
Leinco Technologies’ TMB Microwell Substrates exhibit superior kinetic performance, sensitivity and lot to lot
consistency as compared to other vendors. The outstanding shelf life of at least forty two months for the TMB
HRPO Microwell Slow Kinetic Substrate makes this reagent ideal for long term use of the same manufacturing lot.