MemGlow 488 - Fluorogenic Plasma Membrane probe ex 499 em 507 250-1500 slides (MEMBRIGHT Family Probe)

The MemGlow product line consists of bright & non toxic live cell membrane probes. Originally developed as the MEMBRIGHT probes^1-3 MemGlow fluorogenic probes exhibit ideal microscopy characteristics including high specificity, low background, and simple application. MemGlow 488 has been validated with multiple microscopy techniques including epifluorescent (widefield), confocal, 2-photon, and TIRF¹. MemGlow has been confirmed to work in fixed cells, fixed tissue, live cells, and other phospholipid membranes such as extracellular vesicles including exosomes^1-3.

Features and advantages of MemGlow probes:

• Bright and fluorogenic
• Simple staining protocol and low working concentration
• Compatible with live and fixed cell staining (see important technical notes in About Tab)
• Compatible with ex vivo and fixed tissue staining
• Non-toxic probes permit long-term imaging and re-imaging of live cells
• Efficient labeling of filopodia and nanotubes at nanomolar concentrations
• No dye quenching steps required
• Do not alter sample biology
• Utilize cyanine or BODIPY dyes with zwitterionic membrane anchor groups
• Superior to many existing plasma membrane dyes

For more detailed information on using this product see the About Tab

** 2 nmol of MemGlow will produce a 20uM stock solution when reconstituted with 100 ul of anhydrous DMSO

• For an in-depth look at MemGlow probes (part of the MemBright family) view their groundbreaking publication by clicking this link

• Learn about the advances in membrane probes and tools: newsletter

• See what makes MemGlow a superior membrane dye

Turn-on mechanism of MemGlow probes. MemGlow probes are self-quenched nanoparticles until integration with the plasma membrane enables their excitation.

Cytoskeleton's new MemGlow probes, part of the MemBright family, was used to visualize the plasma membrane of live KB cells with a laser scanning confocal microscope set to 5% laser power (488 nm) for 340 frames with 2 scans per frame and continuous illumination for 15 minutes.

MEMBRIGHT is a trademark of CNRS/UNISTRA of France.