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Human SDF2 Baculovirus-Insect cells Overexpression Lysate

Human SDF2 Baculovirus-Insect cells Overexpression Lysate

Item no.	10950-H08BL-300
Manufacturer	Sino Biological
Amount	300 ug
Category	Cell Lines & Cell Culture Cell Lysates
Type	Cell Lysate
Format	Baculovirus-Insect cells
Specific against	Human (Homo sapiens)
Host	Baculovirus-Infected Insect Cells
ECLASS 10.1	32160890
ECLASS 11.0	32160890
UNSPSC	41105330
Alias	10950-H08BL
Similar products	SDF2
Available
Manufacturer - Category	Lysate
Molecule	SDF2
Product Description	Baculovirus-Insect Cells transfected lysate in which Human SDF2 has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer.
Expressed Host	Baculovirus-Insect cells
Preparation Method	Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer	Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality Control Testing	12.5% SDS-PAGE Stained with Coomassie Blue
Recommend Usage	1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200uL pure water and boiled for 2-5 min. 3. Store it at -80C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles. Notes: The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer	In modified RIPA Lysis Buffer
Storage Instruction	Store at -80C. Aliquot to avoid repeated freezing and thawing
Stability	Samples are stable for up to twelve months from date of receipt at -80C
Application notes	WB: Use at an assay dependent dilution. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 300 ug

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