Comparison

Human DPP4 / DPPIV / CD26 Protein European Partner

Manufacturer Sino Biological
Category
Type Proteins
Specific against Human
Format Human Cell
Amount 20ug
Host Human
Item no. 10688-HNCH-20
eClass 6.1 34160400
eClass 9.0 42020190
Available
Molecule
DPP4
Synonym
ADABP, ADCP2, CD26, DPPIV, TP103
Storage
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction
The native mature form of human DPPIV (NP_001926.2) extracellular domain (Asn 29-Pro 766) was expressed and purified.
Expressed Host
Human Cells
Purity
> 70 % as determined by SDS-PAGE
Bio-Activity
1. Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is > 2, 500 pmoles/min/ug.
2. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 10688-HNCH) bound to Spike (HCoV-EMC/2012) (Cat: 40071-V31B) was 20 nM.
3. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 10688-HNCH) bound to Spike (HCoV-EMC/2012) (Cat: 40071-V05B) was 50 nM.
4. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 10688-HNCH) bound to Spike (HCoV-EMC/2012) (ECD, aa 1-1297) (Cat: 40069-V08B) was 33 nM.
5. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 10688-HNCH) bound to Spike-His (aa 1-760) (Cat: 40021-V08H) was 12 nM.
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Asn 29
Molecule Mass
The recombinant human DPPIV exists as the mature active form, and consists of 738 amino acids with a predicted molecular mass of 85.4 kDa. The apparent molecular mass of the glycosylated rhDPPIV is approximately 95 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5
Accession
NP_001926.2
Amino acid sequence
Asn29-Pro766
TAG
N-cleavage

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 20ug
Available: In stock
available

Delivery expected until 6/13/2024 

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