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Purified Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2)

Purified Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2)

Manufacturer	Tonbo Biosciences
Category	Oncology Antibodies By Cell Type Carcinoma Melanoma Adenocarcinoma Flow Cytometry Immunohistochemistry Immunoprecipitation Western Blot Primary Antibodies Electron Microscopy
Type	Antibody Monoclonal
Specific against	Mouse
Clone	M5/114.15.2
Isotype	IgG2b kappa
Format	Purified
Applications	WB, FC, IP, EM, FA, IHCF/IF
Amount	100 ug
Host	Rat
Item no.	70-5321-U100
Targets	HLA-C
eClass 6.1	32160702
eClass 9.0	42030590
Available
Description	The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7 The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.
Alternative Names	MHC II, IA, IE, I-A/E, IA/IE
Gene ID	14961/14969
Citations	Staehli F, Ludigs K, Heinz LX, Segin-Estevez Q, Ferrero I, Braun M, Schroder K, Rebsamen M, Tardivel A, Mattmann C, MacDonald HR, Romero P, Reith W, Guarda G, and Tschopp J. 2012. J. Immunol. 188: 3820-3828. (Flow cytometry) Parra D, Rieger AM, Li J, Zhang Y-A, Randall LM, Hunter CA, Barreda DR, and Sunyer JO. 2012. J. Leukoc. Biol. 91:525-536. (in vitro blocking, Flow cytometry) Scarlett UK, Rutkowski MR, Rauwerdink AM, Fields J, Escovar-Fadul X, Baird J, Cubillos-Ruiz JR, Jacobs AC, Gonzalez JL, Weaver J, Fiering S, and Conejo-Garcia JR. 2012. J. Exp. Med. 209: 495-506. (Immunofluorescence microscopy - frozen tissue) Chen M, Felix K, and Wang J. 2011. J. Immunol. 187: 5684-5692. (in vitro blocking) Busman-Sahay K, Sargent E, Harton JA, and Drake JR. 2011. J. Immunol. 186:6710-6717. (Immunoprecipitation) Ohmura-Hoshino M, Matsuki Y, Aoki M, Goto E, Mito M, Uematsu M, Hakiuchi T, Hotta H, and Ishido S. 2006. J. Immunol. 177:341-354. (Immunofluorescence microscopy - frozen tissue, Immunoprecipitation) Li C, Siemasko K, Clark MR, and Song W. 2002. Int. Immunol. 14: 1179-1191. (Western Blot, Immunoelectron microscopy)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 5/30/2024