Recombinant Human PDGF-AB (Carrier-free)

Platelet-derived growth factor-AB (PDGF-AB) is one of the five dimeric proteins belonging to the PDGF family. Other members are homodimers PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD. The PDGFs are produced by platelets, stored in platelet alpha-granules and are released upon platelet activation. PDGF proteins are generally considered as potent mitogens for connective tissue cells, and can also be chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. PDGF-AB is reported to support angiogenesis in the heart. Two receptors have been identified for the PDGF proteins - PDGFR alpha and PDGFR beta. PDGF-AB is able to bind to both PDGFR alpha and PDGFR beta.

Alpha chain: SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT

Beta chain: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC

The expected ED50 is <= 1 ng/ml, corresponding to a specific activity of >= 1 x 106 units/mg, as determined by the dose-dependent stimulation of BALB/c 3T3 cell proliferation.

Recombinant Human PDGF-AB is made up of one A and one B chain, resulting in a 26.4 kDa disulfide-linked heterodimer consisting of 234 total amino acids.

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See Certificate of Analysis (COA) for lot specific reconstitution information.