Adrenocorticotropic Hormone (ACTH) BioAssay™ ELISA Kit (Rat)

Kits and Assays / Kits-ELISA (Hormones & Steroids), BioAssay™

4°C/-20°C

The Adrenocorticotropic Hormone (ACTH) BioAssay™ ELISA Kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of ACTH in rat serum, plasma and other biological fluids.

Detection Range:
1.28-800pg/ml

Sensitivity:
0.44pg/ml

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components:
*023173A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use
*023173B: Standard, 2x1 vial
023173C: Standard Diluent, 1x20ml
*023173D: Detection Reagent A (green), 1x120ul
*023173E: Detection Reagent B (red), 1x120ul
023173F: Assay Diluent A, 1x12ml
023173G: Assay Diluent B, 1x12ml
023173H: TMB Substrate, 1x9ml
*023173J: Positive Control, 1x1vial, 86.4pg/ml (Lyophilized)
023173K: Stop Solution, 1x6ml
023173L: Wash Buffer, 30X, 1x20ml

Precaution:
The Stop Solution (023173K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability:
Store *023173A, *023173B, *023173D, *023173E and *023173J at -20°C. After reconstitution of *023173J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 023173J: Positive Control:

Intended Use:
The Positive Control is from is from a rat serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics:
The positive control vial contains rat ACTH in rat serum (lyophilized powder). Note: The rat source material used in this product are from healthy animal of SPF standard (specific pathogen-free animals).

Expected Values:
86.4pg/ml

Usage:
Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.

Assay Procedure Summary:
1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well.
3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.