Epiregulin (EREG) BioAssay™ ELISA Kit (Human)

Kits and Assays / Kits-ELISA (Growth Factors: Epidermal Growth Factor), BioAssay™

4°C/-20°C

Epiregulin (EREG) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EREG in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Detection Range:
15.6-1000pg/ml

Sensitivity:
6.2pg/ml

Intra-Assay:
CV<10%

Inter-Assay:
CV<12%

Kit Components:
*024861A: Microtiter Strips, 1x96wells, Pre-coated, ready to use
*024861B: Standard, 2x1vial
024861C: Standard Diluent, 1x20ml
*024861D: Detection Reagent A (green), 1x120ul
*024861E: Detection Reagent B (red), 1x120ul
024861F: Assay Diluent A, 1x12ml
024861G: Assay Diluent B, 1x12ml
024861H: TMB Substrate, 1x9ml
*024861J: Positive Control, 261pg/ml, 1x1vial
024861K: Stop Solution, 1x6ml
024861L: Wash Buffer, 30x, 1x20ml

Precaution:
The Stop Solution (024861K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability:
Store *024861A, *024861B, *024861D, *024861E and *024861J at -20°C. Store all the other components at 4°C. After reconstitution of *024861J, store at 4°C and use within 5 days. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 024861J: Positive Control:

Intended Use:
The control vials are from human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics:
The control vial contain human EREG in human serum (lyophilized powder). Note: The human based plasma used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.

Expected Values:
261pg/ml

Usage:
Dissolve with 150ul standard diluent and let it stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens.
Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.

Assay Procedure Summary:
1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to eac well. Incubate 1 hour at 37°C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour a 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubat 10-20 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.