Glial Fibrillary Acidic Protein (GFAP) BioAssay™ ELISA Kit (Rat)

Kits and Assays / Kits-ELISA, BioAssay™

4°C/-20°C

Glial Fibrillary Acidic Protein (GFAP) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GFAP in rat serum, plasma and other biological fluids.

Detection Range:
15.62-1000pg/ml

Sensitivity:
6.3pg/ml

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components:
*025284A: Microtiter Strips, 1x48 wells, Pre-coated, ready to use
*025284B: Standard, 2x1vial
025284C: Standard Diluent, 1x10ml
025284D: Detection Reagent A (green), 1x60ul
*025284E: Detection Reagent B (red), 1x60ul
025284F: Assay Diluent A, 1x6ml
025284G: Assay Diluent B, 1x6ml
025284H: TMB Substrate, 1x4.5ml
025284K: Stop Solution, 1x3ml
025284L: Wash Buffer, 30X, 1x10ml

Precaution:
The Stop Solution (025284K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability:
Store *025284A, *025284B, *025284D and *025284E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary:
1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.