Lactate Dehydrogenase (LDH) BioAssay™ ELISA Kit (Human)

Kits and Assays / Kits-ELISA (Tissue and Cell Injury), BioAssay™

4°C/-20°C

Lactate Dehydrogenase (LDH) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of LDH in human serum, plasma, tissue homogenates and other biological fluids.

Detection Range:
0.156-10ng/ml

Sensitivity:
0.061ng/ml

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components:
*026416A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use
*026416B: Standard, 2x1vial
026416C: Standard Diluent, 1x20ml
*026416D: Detection Reagent A, 1x120ul
*026416E: Detection Reagent B, 1x120ul
026416F: Assay Diluent A, 1x12ml
026416G: Assay Diluent B, 1x12ml
026416H: TMB Substrate, 1x9ml
*026416J: Positive Control, 4.80ng/ml, 1x1vial
026416K: Stop Solution, 1x6ml
026416L: Wash Buffer, 30X, 1x20ml

Precaution:
The Stop Solution (026416K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Notes on 026416J: Positive Control:

Intended Use:
The positive control is from a human serum sample known to contain tLactate Dehydrogenase. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics:
The positive control vial contains human LDH in human serum (lyophilized powder). Note: The human based serum used in this product was screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.

Expected Values:
4.80ng/ml

Usage:
Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.

Storage and Stability:
Store *026416A, *026416B, *026416D, *026416E and *026416J at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary:
1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.