Myostatin (MSTN) BioAssay™ ELISA Kit (Human)

Kits and Assays / Kits-ELISA, BioAssay™

4°C/-20°C

The Human Myostatin (MSTN) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MSTN in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Detection Range:
0.78 -50ng/ml

Sensitivity:
0.28ng/ml

Precision:
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Test Principle:
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MSTN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MSTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MSTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MSTN in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Kit Components:
*026979A: Microtiter Plate, 1x96 wells, Pre-coated; ready to use
*026979B: Standard, 2x1vial
026979C: Standard Diluent, 1x20ml
*026979D: Detection Reagent A, 1x120ul
*026979E: Detection Reagent B, 1x120ul
026979F: Assay Diluent A, 1x12ml
026979G: Assay Diluent B, 1x12ml
026979H: TMB Substrate, 1x9ml
026979K: Stop Solution, 1x6ml
026979L: Wash Buffer, 30x, 1x20ml

Storage and Stability:
Store *026979A, *026979B, *026979D and *026979E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.