GFAP, Blocking Protein (Glial Fibrillary Acidic Protein)

Blue Ice

49, 5

Supplied as a liquid.

Blocking Protein for G2032-27J.

Human GFAP is a 49.7kD protein (432aa) expressed in astrocytes. GFAP is an intermediate filament protein and acts as an intracellular structural component of the cytoskeleton. During embryonic and fetal life, GFAP is also expressed by radial glial cells of the CNS. Various mutations of the GFAP gene in humans produce Alexander's disease, one of the leukodystrophies. Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP.

Source:
Purified Bovine GFAP protein

AA Sequence:
MERRRVTSATRRSYVSSSEMVVGGRRLGPG TRLSLARMPP PLPARVDFSLAGALNSGFKETRASERAEMM ELNDRFASYI EKVRFLEQQN KALAAELNQLRAKEPTKLAD VYQAELRELR LRLDQLTANS ARLEVERDNL AQDLGTLRQK LQDETNQRLE AENNLAAYRQ EADEATLARLDLERKIESLE EEIRFLRKIH EEEVRELQEQ LAQQQVHVEM DVAKPDLTAALREIRTQYEA VASSNMHEAE EWYRSKFADL NDAAARNAELLRQAKHEAND YRRQLQALTC DLESLRGTNESLERQMREQE ERHAREAASY QEALARLEEE GQSLKDEMAR HLQEYQDLLN VKLALDIEIA TYRKLLEGEE NRITIPVQTF SNLQIRETSL DTKSVSEGHL KRNIVVKTVE MRDGEVIKESKQEHKDVM

Molecular Weight:
~49.5kD

Applications:
Suitable for use in ELISA and Antibody Blocking. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.