RNF4, Recombinant, Human, aa1-190, His-Tag (E3 Ubiquitin Ligase RNF4, RES4-26, SLX5, SNURF)

Blue Ice

23, 7

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.5, 0.2M sodium chloride, 1mM DTT, 30% glycerol.

E3 ubiquitin ligase RNF4, also known as RNF4, contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein.

Source:
Recombinant protein corresponding to aa1-190 from human RNF4 at N-terminal, fused to His-tag expressed in E.coli.

Molecular Weight:
~23.7kD (213aa)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGS VIVDERRRPR RNARRLPQDH ADSCVVSSDD EELSRDRDVY VTTHTPRNAR DEGATGLRPS GTVSCPICMD GYSEIVQNGR LIVSTECGHV MSTRKRR GGAINSRQAQ KRTREATSTP EISLEAEPIE LVETAGDEIV DLTCESLEPV VVDLTHNDSV FCSQCLRDSL KNANTCPTCR KKINHKRYHP IYI

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.