sRANK Ligand,Recombinant,Rat (Tumor Necrosis Factor Ligand Superfamily Member 11,Osteoclast Differentiation Factor,ODF,Osteoprotegerin Ligand,OPGL,Receptor Activator of Nuclear Factor kappa-B Ligand,RANKL,TNF-related Act

Molecular Biology / MB-Growth Factors-RANK

-20°C

Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.5. No preservatives added. Reconstitute with sterile ddH2O to a concentration of 0.1mg/ml.

RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage.

Recombinant rat sRANKL is a 19.4kD polypeptide comprising the TNF homologous region of RANKL (174aa residues).

Biological Activity:
Determined by its ability to induce NF-kB in RAW264.7 cells in the absence of any cross-linking. The expected ED50 for this effect is 10-25ng/ml.

Amino Acid Sequence:
PAMMEGSWLD VARRGKPEAQ PFAHLTINAA DIPSGSHKVS LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPA DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISVQV SNPSLLDPDQ DATYFGAFKV QDID

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

sRANK Ligand, Recombinant, Rat (Tumor Necrosis Factor Ligand Superfamily Member 11, Osteoclast Differentiation Factor, ODF, Osteoprotegerin Ligand, OPGL, Receptor Activator of Nuclear Factor kappa-B Ligand, RANKL, TNF-related Activation-induced Cytok