Caspase 1 (CASP1) Recombinant, Porcine

Blue Ice

21, 4

Supplied as lyophilized powder from PBS, pH 7.4, 5% trehalose, 0.01% sarcosyl. Reconstitute with sterile PBS, pH 7.4 to a concentration of 0.1-1mg/ml. Do not vortex.

Recombinant protein corresponding to
aa120-297 with an N-Terminal HisTag
from porcine Caspase 1 expressed in E. coli Swiss/UniProt Accession: Q9N2I1.

Purity:
≥95%

Amino Acid Sequence:
MGHHHHHHSGSEF-N PVKPASSEPR GSLKLCPPDI AQRLWKEKSA EIYPIMGKSI RTRLALIICN TEFENLPRRD GADVDIRDMK ILLEDLGYSV DVRENLTASD MAIELKAFAA RPEHKSSDST FLVLMSHGIQ AGICGKKYSE EVPDVLEVNT VFQILNTLNC PSLKDKPKVI IIQACRGEKQ GVVWIKD

Predicted Molecular Weight:
21.1kD

Accurate Molecular Mass:
21kD as determined by SDS-PAGE reducing conditions

Predicted Isoelectric Point:
6.7

Subcellular Location:
Secreted

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.