ATP5F1, Recombinant, Human, aa83-256, His-Tag (ATP Synthase Subunit B, ATP Synthase Subunit B, Mitochondrial)

Blue Ice

22, 6

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.4M urea, 10% glycerol.

ATP5F1 is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8).

Source:
Recombinant corresponding to aa83-256 from human ATP5F1 protein, fused to His-tag at N-terminus, expressed in E.coli.

Molecular Weight:
~22.6kD (197aa)

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Applications:
Suitable for use in SDS-PAGE. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.