NRGN, Recombinant, Human, aa1-78, His-Tag (Neurogranin, Protein Kinase C Substrate)

Blue Ice

10

Supplied as a liquid in 20mM Tris-HCl, pH 7.0, 0.1mM PMSF, 1mM EDTA, 30% glycerol.

NRGN, also known as Neurogranin, is a calmodulin-binding protein expressed exclusively in the brain, particularly in dendritic spines, and participating in the protein kinase C signaling pathway. This protein is the main postsynaptic protein regulating the availability of calmodulin, binding to it in the absence of calcium. Phosphorylation by protein kinase C lowers its binding ability.

Source:
Recombinant protein corresponding to aa1-78 from human NRGN, fused to His-tag at N-terminus, expressed in E. coli.

Molecular Weight:
~10kD (101aa), confirmed by MALDI-TOF

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMDCCTEN ACSKPDDDIL DIPLDDPGAN AAAAKIQASF RGHMARKKIK SGERGRKGPG PGGPGGAGVA
RGGAGGGPSG D

Applications:
Suitable for use in SDS-PAGE. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.