VEGF165, Recombinant, Human, aa1-191, His-Tag (Vascular Endothelial Growth Factor A)

Blue Ice

23, 1

Supplied as a liquid in PBS, pH 7.4, 1mM DTT, 0.1mM PMSF, 10% glycerol.

Vascular endothelial growth factor A, also known as VEGF, is growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. This protein binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Source:
Recombinant protein corresponding to aa1-191 from human VEGF165, fused to His-tag at C-terminus, expressed in Insect cell.

Molecular Weight:
~23.1kD (197aa)

Applications:
Suitable for use in SDS-PAGE. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.