Apolipoprotein E, Recombinant, Rabbit, aa19-311, His-Tag (APOE)

Molecular Biology / MB-Lipoproteins

-20°C

Supplied as a liquid in Tris-HCl, pH 8.0, 1mM EDTA, 50% glycerol.

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.

Source:
Recombinant protein corresponding to aa19-311 from rabbit APOE, fused to His-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E. coli.

Amino Acid Sequence:
QT EQEVEVPEQA RWKAGQPWEL ALGRFWDYLR WVQSLSDQVQ EELLSSQVTQ ELTMLMEETM KEVKAYKSEL EEQLSPMAQE HRARLSKELQ VAGALEADME DVCNRLAQYR GEAQAMLGQS TEELARAFSS HLRKLRKRLL RDAEDLQKRM AVYGAGAREG AERGVSAVRE RLGSRLERGR LRVATVGTLA GRPLRERAQA WGERLRGHLE EVGSRARDRL NEVREQVEEV RVKVEEQAPQ MRLQAEAFQA RLKSWFEPLV EDMQRQWAGL VEKLQAAMPS KAPAAAPIEN Q

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.