Insulin, Recombinant, Rat

Molecular Biology / MB-Hormones, Steroids

-20°C

Highly Purified

Recombinant protein corresponding to aa25~54 and aa90-110 linked by a polypeptide linker (GGGGS), (Accession # P01323), with two N-terminal Tags, His-tag and T7-tag expressed in E. coli.

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF - FVKQHL CGSHLVEALY LVCGERGFFY TPMSGGGGSG IVDQCCTSSIC SLYQLENYCN

Predicted Molecular Weight:
9.9kD

Subcellular Location:
Secreted

Predicted isoelectric point:
6.7

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, and SDS-PAGE. ther Applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Included Protein Molecular Weight Marker:
168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed.
MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1m sodium azide, 0.01% bromo-phenol bl 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20 Stable for 6 months after receipt.
Note:
Thermal stability is described by the los rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradati or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Storage and Stability:
Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.