IL-33, Recombinant, Mouse, His-Tag, aa109-266 (Interleukin-33, IL-1F11, NF-HEV)

Blue Ice

20

Supplied as a liquid from a 0.2um-filtered solution in 55mM TRIS-Cl, pH 8.2, 150mM sodium chloride.

Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kD human IL33 is converted by CASP1 to a 18kD protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38 (MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.

Recombinant protein corresponding to aa109-266 of mouse IL-33, fused to His-Tag at C-terminal, expressed in E. coli.

Molecular Weight:
~20kD (SDS-PAGE)

Biological Activity:
Activates human ST2-dependent NF-kappaB pathway.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.