Interferon Gamma, Recombinant, Canine (IFNg)

Blue Ice

20, 7

Supplied as lyophilized powder from 100mM NaHCO3, 500mM sodium chloride, pH8.3, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% trehalose, Proclin300.

IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-gamma signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-gamma stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Recombinant canine IFN-gamma is a 20.7kD protein..

Recombinant protein corresponding to amino acid 24-166 with two N-Terminal Tag, His-Tag and T7-Tag from canine IFNg (P42161), expressed in E. coli.

Molecular Weight:
~20.7kD

Predicted Isoelectric Point:
9.9

Subcellular Location:
Secreted

Applications:
Suitable for use in SDS-PAGE, ELISA, Western Blot, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QAMFFKE IENLKEYFNA SNPDVSDGGS LFVDILKKWR EESDKTIIQS QIVSFYLKLF DNFKDNQIIQ RSMDTIKEDM LGKFLNSSTS KREDFLKLIQ IPVNDLQVQR KAINELIKVM NDLSPRSNLR KRKRSQNLFR GRRASK

Included Protein Molecular Weight Marker:
168631: Unstained Protein Molecular Weight Marker, 10-70kD
Suppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Ready to use; no need to heat, dilute or add reducing agents before use.

Protein Bands: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD.
Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier.

Usage: Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20°C will go back into solution. Do not boil! Load the following volumes on SDS-PAGE gel:
Mini gels: 3-5ul/well
Large gel: 7ul/well
The loading volumes listed above are recommended for gels with thickness of 0.75-1mm. The loading volume should be doubled for 1.5mm thick gels.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Storage and Stability:
Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.