Tris Hydrochloride (Tris HCl)

RT

157, 6

White, crystalline powder

Buffering agent in incubation mixtures. It has also been used as a component of lysis and TE (Tris-EDTA) buffer. One of the biological buffers.

Synonyms:
Tris(hydroxymethyl)aminomethane HCl;  2-Amino-2-(hydroxymethyl)-1, 3-propanediol HCl; Tromethamine HCl

CAS No:
C4H11NO3•HCl

Molecular Formula:
C4H11NO3•HCl

Molecular Weight:
157.60

Purity:
≥99%

Appearance:
White, crystalline powder

pH (1.0M, H2O):
3.5-5.0

pKa (20°C:)
8.3 ± 0.15

Absorbance (260nm):
≤0.01

Absorbance (280nm):
≤0.05

Solubility (1M, H2O):
Colorless, clear, complete

Loss on Drying:
≤0.3%

Residue on Ignition:
≤0.1%

Melting Point:
150-154°C

Heavy Metals (Pb):
≤0.0005%

Iron (Fe):
≤0.005%

Insoluble Matter:
≤<0.005%

RNase:
None Detected

DNase:
None Detected

Protease:
None Detected

Storage:
RT